A real-time RT-PCR assay intended for detecting nucleic acids from the SARS-CoV-2 virus and four of the most common strains of influenza (B Yamagata, B Victoria, A H1N1 and A H3N2).
Multiplex RT-qPCR assay
Multiplex RT-qPCR assay
Introduction of CoviFlu™
PentaBase’s CoviFlu™ Multiplex RT-qPCR diagnostic assay is a real-time RT (reverse transcriptase) PCR assay intended for the detection of nucleic acids from the SARS-CoV-2 virus and the four most common strains of influenza (B Yamagata, B Victoria, A H1N1, and A H3N2). SARS-CoV-2 and influenza RNA can be found in the liquid from the upper or lower respiratory tracts of infected individuals. Samples can be obtained by nasopharyngeal or oropharyngeal swabs or from sputum.
The symptoms of SARS-CoV-2 have a lot in common with the symptoms of influenza, which can be difficult for physicians and clinicians to differentiate and diagnose correctly. PentaBase’s influenza COVID-19 multiplex RT-qPCR test is intended to identify the presence of SARS-CoV-2 virus or influenza RNA by a fast and reliable method that rules out either COVID-19 or Influenza.
Available in the following formats:
Dispense Ready (DR)
Ready-To-Use (RTU)
TECHNOLOGY AND REAGENTS
The CoviFlu™ Multiplex RT-qPCR assay combines real-time PCR with PentaBase’s novel and selective technologies comprising both standard synthetic oligonucleotides as well as proprietary modified synthetic oligonucleotides such as HydrolEasy™, EasyBeacon™ probes, and SuPrimers™ for specific and sensitive amplification.
The technology is applicable on standard real-time PCR equipment using standard procedures. Pentabase-modified oligos contain synthetic DNA analogues comprising a flat heteroaromatic, hydrophobic molecule and a linker. These modifications are inserted into the oligonucleotides at fixed positions during synthesis. Using the CoviFlu™ assay, the presence of virus RNA in a sample can be detected quickly, sensitively, and selectively by real-time RT-PCR analysis.
Approved Instruments for Use of CoviFlu™ Assay
PentaBase systems for CoviFlu™ Assay
CoviFlu™ Multiplex RT-qPCR assay is currently designed to run on BaseTyper™ (PentaBase PCR instrument). Optimal PCR profiles are developed for each validated instrument. More instruments and settings will be added when they are validated.
