PentaBase is offering a high quality dual-labelled DNA probes and improved DNA probes with a broad range of different quenchers and fluorophores.
DUAL-LABELED DNA PROBES
DUAL-LABELED DNA PROBES
INTRODUCTION
PentaBase offers high-quality dual-labeled DNA probes and improved DNA probes with a broad range of quenchers and fluorophores. Dual-labeled probes typically contain a quencher at the 3′-end and a fluorophore at the 5′-end but can also contain the fluorophore and/or quencher internally in the sequence. For example, we offer two types of dual-labeled probes for Real-Time PCR:
EasyBeacons™ Probes
HydrolEasy™ Probes
BENEFITS OF HYDROLEASY™ PROBES
HydrolEasy™ probes are hydrolysis probes with significantly better signal-to-noise ratio, higher specificity, and higher sensitivity than conventional probes. When the HydrolEasy probe is intact, the fluorophore and the quencher are, in contrast to “standard” TaqMan® probes, kept in close proximity by hydrophobic interactions between PentaBases. During the amplification reaction, the probe hybridizes with the target, and the exonuclease activity of the polymerase cleaves the fluorophore from the probe, whereby a strong fluorescence signal is generated.
HydrolEasy™ Probes are developed to provide TaqMan® users, TaqMan®MGB users, and other hydrolysis probes with an improved alternative for better signal-to-noise ratio, higher specificity, and sensitivity. A HydrolEasy™ Probe is a dual-labeled probe with a standard fluorophore in the 5’ end and a quencher in the 3’ end. We added our proprietary technology to improve sensitivity, specificity, affinity, and signal-to-noise ratio.
BENEFITS OF EasyBeacons™
EasyBeacons™ are nuclease-resistant probes developed by PentaBase to be used in 3-step Real-Time PCR and situ hybridizations. Like Molecular Beacons, EasyBeacons™ are quenched when the probes are unbound but fluoresce at hybridization to a target sequence. However, unlike Molecular Beacons, EasyBeacons™ do not require an internal stem to be quenched efficiently. The PentaBases in EasyBeacons™ will, by having hydrophobic interactions with each other in the unbound probe, ensure that the quencher and fluorophore are kept in close proximity, resulting in an efficiently quenched probe with low background fluorescence. This mechanism of action is temperature independent and thereby significantly increases the working temperature window of the probes and the ease of use.
EasyBeacons™ Mechanism principles
Due to EasyBeacons™ nuclease resistance, they can also be used for end-point detection or verification of your Real-Time signal. This is a valuable feature, especially if you want to discriminate between almost similar genetic sequences like SNPs or if you want quality control on your quantification data. This is a valuable feature, especially for genotyping similar genetic sequences like SNPs and CpG methylation status.
Benefits of BaseProbes™
BaseProbes™ is a universal master mix comprising one to four qPCR probes. The system combines the versatility of SYBRTM Green-type detection with the multiplex capability of molecular probes. The BaseProbes™ technology is compatible with standard qPCR configuration and suitable for customization.
BaseProbes™ detection technology is an alternative to SYBRTM green-based analysis of SNPs, insertion/deletion variations, and semi-quantitative or identification as used in, e.g., infectious disease.
Key features of BaseProbes™ :
Migrate your current assay easily
Inexpensive NGS mutation confirmation
Allow for internal control
