Patients with microsatellite instable tumors benefit from specific treatments more than others. Therefore, early detection of instability becomes important. MicroSight MSI allows for such a result in less than two hours and provides a precise, personalised and, most importantly, beneficial treatment plan

World Cancer Research Fund’s data (2020) showed that 10.7% (3'rd most occurring) of all new global cancers diagnosed were colorectal cancer. PentaBase’s MicroSight MSI is a critical analysis of microsatellite instability commonly found, among others, within colorectal cancer patients.
The MicroSight MSI assay is a highly sensitive analysis of human DNA samples that gives insight into the instability within five different biomarkers (mononucleotide microsatellite loci). This makes it a careful and precise stratification tool to assist with treatment planning. The analysis is based on validated methods, withholds flexibility through paired or universal references (UR), is incredibly cost-efficient due to the minimal hands-on time needed, and is created to sincerely strive for optimal caring of its end-users.
Advantages of MicroSight® MSI:
Results in less than two hours
Low risk of cross contamination
Objective analysis – No biased data interpretation
One instrument analysis – minimal hands-on time

One of the fastest MSI Analysis in the world
We deliver the best possible solutions for patients’ health situations. Consequently, MicroSight MSI assists in making the proper treatment plan, the first time around. Therefore, it provides results in less than two hours, flexibility through two versions, precise analysis of up to seven patients simultaneously and does so all in one instrument.
2
Hour result time
7
Patients
2
Versions
1
Instrument
Paired Samples
MicroSight MSI offers an adaptive analysis of either paired or unpaired samples. A biopsy from a tumor and cell-free DNA from the patient are placed into two sets of 5-well PCR strips in the paired samples analysis. The strips are analyzed on our efficient real-time PCR machine, BaseTyper™ 48, where each run allows for quick analysis of 4 different patients simultaneously (4 sets of 5-well strips).
- The unique analysis of each patient’s specific DNA constitution.
- Needs only < 1 positive microsatellite
- A quick, simultaneous 4-patient analysis
Instructions of Use:

Unpaired Samples
The unpaired samples analysis only needs tumor DNA from the patient, which is then compared to a universal reference. The tumor DNA is placed into 5-well strips, but because of the universal reference that only needs one 5-well strip, each run allows for quick analysis of seven different patients simultaneously (7x5-well strips + 1 UR 5-well strip).
- High throughput because of universal reference
- Needs a minimum of 3 positive microsatellites
- A quick, simultaneous 7-patient analysis
Instructions of Use:

Product Information
PentaBase systems for analysis
The MicroSight® MSI 1-step HRM analysis is designed and validated for usage on BaseTyper™48.4 (PentaBase PCR instrument).
Fast and accurate real-time PCR
Minimal hands-on time required
Small, compact instrument with mobile capabilities


Order MicroSight® MSI 1-step HRM analysis
Are you interested in our assays for analysing the stability/instability of five mono-nucleotide microsatellite loci? Find a local distributor, become our distributor or order your assay at PentaBase directly.