Patients with microsatellite instable tumors benefit from specific treatments more than others. Therefore, early detection of instability becomes important. MicroSight MSI allows for such a result in less than two hours and provides a precise, personalised and, most importantly, beneficial treatment plan
World Cancer Research Fund’s data (2020) showed that 10.7% (3'rd most occurring) of all new global cancers diagnosed were colorectal cancer. PentaBase’s MicroSight MSI is a critical analysis of microsatellite instability commonly found, among others, within colorectal cancer patients.
The MicroSight MSI assay is a highly sensitive analysis of human DNA samples that gives insight into the instability within five different biomarkers (mononucleotide microsatellite loci). This makes it a careful and precise stratification tool to assist with treatment planning. The analysis is based on validated methods, withholds flexibility through paired or universal references (UR), is incredibly cost-efficient due to the minimal hands-on time needed, and is created to sincerely strive for optimal caring of its end-users.
Advantages of MicroSight® MSI:
Results in less than two hours
Low risk of cross contamination
Objective analysis – No biased data interpretation
One instrument analysis – minimal hands-on time
Paired Samples
MicroSight MSI offers an adaptive analysis of either paired or unpaired samples. A biopsy from a tumor and cell-free DNA from the patient are placed into two sets of 5-well PCR strips in the paired samples analysis. The strips are analyzed on our efficient real-time PCR machine, BaseTyper™ 48, where each run allows for quick analysis of 4 different patients simultaneously (4 sets of 5-well strips).
Instructions of Use:
Unpaired Samples
The unpaired samples analysis only needs tumor DNA from the patient, which is then compared to a universal reference. The tumor DNA is placed into 5-well strips, but because of the universal reference that only needs one 5-well strip, each run allows for quick analysis of seven different patients simultaneously (7x5-well strips + 1 UR 5-well strip).
Instructions of Use:
PentaBase systems for analysis
The MicroSight® MSI 1-step HRM analysis is designed and validated for usage on BaseTyper™48.4 (PentaBase PCR instrument).
Fast and accurate real-time PCR
Minimal hands-on time required
Small, compact instrument with mobile capabilities
