Patients with microsatellite instable tumors benefit from specific treatments more than others. Therefore, early detection of instability becomes important. MicroSight MSI allows for such a result in less than two hours and provides a precise, personalised, and most importantly, beneficial treatment plan
Microsatellite instability (MSI) is a genomic alteration in the length of microsatellites provoked by a deficient DNA mismatch repair system. MSI is known to occur in several types of cancer e.g., colorectal cancer (CRC), which accounts for 10.7% of newly diagnosed cancers globally (3’rd most occurring) according to World Cancer Research Fund’s data (2020). Identification of MSI in tumours is important as a predictive factor of response to different therapies, in particular to immune checkpoint inhibitors, and accordingly the need for MSI analysis of these patients has significantly increased. The MicroSight® MSI 1-step HRM Analysis assay analyses five well-described mononucleotide microsatellite sequences: BAT25, BAT26, NR22, NR24, and MONO27, by Real-Time PCR and HRM. The assay evaluates microsatellite length of patient tumour DNA to patient non-tumour DNA or a universal reference, which makes it a valuable stratification tool for assisting cancer treatment planning. After the Real-Time PCR and HRM, a semi-automatic analysis will be made by the instrument software giving objective results in less than 2 hours.
Advantages of MicroSight® MSI:
Results in less than two hours
Minimal hands-on time
Objective analysis – No biased data interpretation
One instrument analysis – Low risk of cross contamination
Paired Samples
MicroSight® MSI 1-Step HRM Analysis assay offers an adaptive analysis of either paired or unpaired samples. Purified patient tumor and non-tumor DNA is added to individual 5-well PCR strip in the paired samples analysis. The strips are analysed in a BaseTyper™ 48.4 Real-Time PCR Instrument, where each run allows for quick analysis of up to 4 different patients simultaneously (4 sets of 2x5-well strips).
Instructions for Use:
Unpaired Samples
The unpaired sample analysis by MicroSight® MSI 1-Step HRM Analysis assay only needs tumour DNA from the patient, which is then compared to a Universal Reference DNA included in the kit. This allows for analysis of up to seven different patients simultaneously per Real-Time PCR run (7x patient 5-well strips + 1 Universal Reference 5-well strip).
Instructions for Use:
PentaBase systems for analysis
The MicroSight® MSI 1-Step HRM Analysis assay is designed and validated for usage with the BaseTyper™48.4 Real-Time PCR Instrument
Fast and accurate qPCR
Minimal hands-on time required
Small, compact instrument with mobile capabilities
