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MicroSight® MSI 1-step HRM Analysis for Microsatellite Instability

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Laboratory analysis at PentaBase
1-step HRM Analysis

MicroSight® MSI

Patients with microsatellite instable tumors benefit from specific treatments more than others. Therefore, early detection of instability becomes important. MicroSight MSI allows for such a result in less than two hours and provides a precise, personalised, and most importantly, beneficial treatment plan

Specifications How to order
Intended use of MicroSight® MSI 1-step HRM analysis

Microsatellite instability (MSI) is a genomic alteration in the length of microsatellites provoked by a deficient DNA mismatch repair system. MSI is known to occur in several types of cancer e.g., colorectal cancer (CRC), which accounts for 10.7% of newly diagnosed cancers globally (3’rd most occurring) according to World Cancer Research Fund’s data (2020). Identification of MSI in tumours is important as a predictive factor of response to different therapies, in particular to immune checkpoint inhibitors, and accordingly the need for MSI analysis of these patients has significantly increased. The MicroSight® MSI 1-step HRM Analysis assay analyses five well-described mononucleotide microsatellite sequences: BAT25, BAT26, NR22, NR24, and MONO27, by Real-Time PCR and HRM. The assay evaluates microsatellite length of patient tumour DNA to patient non-tumour DNA or a universal reference, which makes it a valuable stratification tool for assisting cancer treatment planning. After the Real-Time PCR and HRM, a semi-automatic analysis will be made by the instrument software giving objective results in less than 2 hours.

Advantages of MicroSight® MSI: 

Results in less than two hours

Minimal hands-on time

Objective analysis – No biased data interpretation

One instrument analysis – Low risk of cross contamination

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One of the fastest MSI Analysis in the world

MicroSight® MSI 1-step HRM Analysis assists in making the proper treatment plan for cancer patients. It; provides results in less than two hours, has flexibility through two versions, gives precise analysis of up to seven patients simultaneously, and does so all in one instrument.

Other Assays

2

Hour result time

2

Versions (Paired or Unpaired)

4 or 7

Patients

1

Instrument

Flexible Use for diagnostics

Paired Samples
MicroSight® MSI 1-Step HRM Analysis assay offers an adaptive analysis of either paired or unpaired samples. Purified patient tumor and non-tumor DNA is added to individual 5-well PCR strip in the paired samples analysis. The strips are analysed in a BaseTyper™ 48.4 Real-Time PCR Instrument, where each run allows for quick analysis of up to 4 different patients simultaneously (4 sets of 2x5-well strips).

  • The unique analysis of each patient’s specific DNA constitution
  • Needs only 2 positive microsatellite loci for identification
  • A quick, simultaneous 4-patient analysis

Instructions for Use:

Download IFU
Flexible Use for diagnostics

Unpaired Samples
The unpaired sample analysis by MicroSight® MSI 1-Step HRM Analysis assay only needs tumour DNA from the patient, which is then compared to a Universal Reference DNA included in the kit. This allows for analysis of up to seven different patients simultaneously per Real-Time PCR run (7x patient 5-well strips + 1 Universal Reference 5-well strip).

  • High throughput by use of Universal Reference DNA
  • Needs 3 positive microsatellite loci 
  • A quick, simultaneous 7-patient analysis
  • No requirement for matched uninvolved biopsy

Instructions for Use:

Download IFU


Product Information

Please refer to the IFU for acceptable sample type and collection device combinations.
Formats
Paired & Unpaired (Universal Reference) 
Loci
BAT25, BAT26, NR22, NR24 & MONO27
No. of Reactions
Box of 16 reactions 
Configuration
Five simplexes as ready-to-use assays in 5-well PCR strips 
Shipment Condition
≤-20°C or on Dry ice
Storage
-20°C
Instrumentation Required
Nucleic Acid Extraction System & BaseTyper™ 48.4 Real-Time PCR instrument
Result Time
Less than 2 hours
Concentration of Genomic DNA
1-10 ng/µL for qPCR
Reagents and Materials
Pre-aliquoted 0.2 mL PCR strips, Universal reference (8 reactions), Positive control (2 reactions) & Negative control (2 reactions)
Validated Extraction methods
QIAamp DNA FFPE Tissue Kit, BaseRelease™ FFPE & BasePurifier™ FFPE
Intended use
CE IVD
Approved Instrument for Use of MicroSight® MSI 1-step HRM analysis

PentaBase systems for analysis

The MicroSight® MSI 1-Step HRM Analysis assay is designed and validated for usage with the BaseTyper™48.4 Real-Time PCR Instrument

Fast and accurate qPCR

Minimal hands-on time required

Small, compact instrument with mobile capabilities

BaseTyper™ 48.4
BaseTyper PCR equipment
Distributor celloxa Distributor Anachem Greec/Cyprus Distributor Dynex Distributor AmpliTech Distributor Ridacom Distributor Resnova
Sponsor and collaborator
How to order

Order MicroSight® MSI 1-step HRM Analysis assay

Are you interested in our assays for analysing the stability/instability of five mono-nucleotide microsatellite loci? Find a local distributor, become our distributor or order your assay at PentaBase directly.

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