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Friday 12. Aug 2022 by Aminat Makhauri

InfectionDetect-MPOX qPCR assay – A Danish developed qPCR assay for Monkeypox

Friday 29. Jul 2022 by Aminat Makhauri

MicroSight® MSI 1-step HRM Analysis for Microsatellite Instability

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OLIGONUCLEOTIDE | PRIMERS | PROBES

Our proprietary technology

PentaBase’s proprietary technology, Intercalating Nucleic Acid® (INA®), is a hydrophobic base analog that is incorporated into oligonucleotides without replacing any nucleotide in the oligonucleotide sequence. The integrated INA® enhances the stacking effect of the DNA helix, which increases affinity, sensitivity and specificity of the oligonucleotide.

Purification | Quality Control

Reverse-phase HPLC purifies all PentaBase probes to give the purest products. Primers are also purified by reverse-phase HPLC and desalting and removing the final DMT group. Desalting removes residual by-products from the different processes.

Every probe from PentaBase is strictly controlled for quality and fluorescence potential in an affinity test. To do this test, we synthesize a complementary oligo target and run a melting curve from 95°C to 35°C. The figure shows the result of such a test. The red curve shows the probe in the presence of its complementary target, and the yellow line shows the probe without a target. Halfway down, the dip in fluorescence of the red line corresponds to the melting temperature of the duplex. The exact melting temperature of the probe-target duplex is found by calculating the inverted derivative of the curve.

Explore our PCR Technology

We use our PCR technology to create custom DNA oligos, primers, probes and NGS adapters. We guarantee superior quality and results in your work.

Custom DNA oligos

Custom Oligo

TECHNOLOGY

BaseBlockers™

PROBES

Easybeacon™

PROBES

Hydroleasy™

Universal Master Mix

Baseprobes™

TECHNOLOGY

(Su)Primers™

NEXT-GENERATION SEQUENCING

PentAdapter™

TECHNOLOGY

Fluorescent Probes

PentaBase laboratory

Contact us

If you have further questions regarding our technology or service, you can get in contact with our product specialist, who will be able to assist you.

 

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