Assays | Cancer diagnosis and stratification

PlentiPlex_™ Mastocytosis

PlentiPlex™ KIT Mastocytosis qPCR Assay provides a fast and easy diagnosis of the Mastocytosis condition, more specifically the detection of the KIT D816V mutation

Specifications How to order

INTENDED USE OF PlentiPlex™ KIT Mastocytosis

PlentiPlex™ Mastocytosis assay is intended for the detection of the KIT D816V mutation, which is among the WHO criteria for the diagnosis of mastocytosis. PlentiPlex™ Mastocytosis Assay utilises PentaBase’s in-house production of custom DNA molecules based on our DNA chemistry, known as Intercalating Nucleic Acid (INA). This technology provides an analysis that discovers and identifies if the patient has a disorder characterised by the growth and accumulation of neoplastic mast cells in various organs, most commonly in the skin.

Advantages of PlentiPlex™ Mastocytosis:

The Assay follows WHO criteria

Applicable for large biopsies

2 formats (Ready-to-use & Dispense ready)

Enables further personalised cancer treatment

Instructions for Use: PlentiPlex™ Mastocytosis

PUT YOUR HEALTH FIRST

Highly sensitive Mastocytosis diagnosis

If provided with sufficient input of DNA of sufficient quality, the assay can detect 0.01% of KIT D816V mutated gDNA in a background of wild-type gDNA. As a result, it offers two versions (RTU and DR), and two configurations (Simplex and Multiplex) all in one single solution: The PlentiPlex™ Mastocytosis

Other Assays

Solution

Hour result time

Versions

Instrument

Product information

Format

Ready-to-use and Dispense-ready

Loci

KIT D816V allele

Design

Reference assay and mutation assay

Equipment required

Template DNA (extracted mutant gDNA)
Applied Biosystems QuantStudio™ Real-Time PCR instrument

Shipment

With dry ice or -80°C ice packs

Storage

Range between -20°C and -80°C

Content

Ready-to-use: 12 or 60 PCR tube strips
In pre-aliquoted 0.1 ml or 0.2 ml PCR strips or tubes suitable for most standard 96-well plate format PCR instruments.
Dispense ready: 20 or 50 reactions

Result time

≤ One and a half hour

Detection sensitivity

0.01% limit of detection of KIT D816V mutation in a wild type gDNA background

Intended use

CE IVD

Approved Instruments for Use of PlentiPlex™ Mastocytosis CE IVD assays

PentaBase systems for analysis

The PlentiPlex™ Mastocytosis CE IVD assays are now designed and validated to be used on BaseTyper™48.4(PentaBase PCR instrument) and the following instruments:

Rotor-Gene (Qiagen)

MX3000P/3005P (Agilent)

LightCycler® 96 + 480 (Roche)

CFX96TM (Bio-Rad)

ABI 7500/7900HT (Applied Biosystems)

MIC (Bio Molecular Systems)

MyGo Pro (IT-IS Life Science)

BaseTyper™48

How to order

PlentiPlex_™ KIT Mastocytosis qPCR Assay

Are you interested in our assays for the diagnosis of the Matocytosis condition? Find a local distributor, become our distributor, or order your assay at PentaBase directly.

Order with PentaBase Order from your local distributor Become distributor

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World´s first DNA methylation assay without pre-treatment

PlentiPlex_™ Mastocytosis

Highly sensitive Mastocytosis diagnosis

Product information

PlentiPlex_™ KIT Mastocytosis qPCR Assay