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InfectionDetect-MPOX qPCR assay – A Danish developed qPCR assay for Monkeypox

Friday 29. Jul 2022 by Aminat Makhauri

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msi analysis

Oligonucleotides

Our proprietary and unique INA™ base analogue technology allows us to produce oligonucleotides with increased target affinity and specificity compared to standard DNA oligonucleotides

Custom Oligo Technology Primers
Custom Oligos

Our custom DNA oligos are produced with thorough and careful quality control and quick turnaround time. We ensure quality oligos!
Order your custom oligos using the button below for fast and easy ordering. Write "multiple sequences" if you wish to order more than one oligo at a time. This makes the process easier.

If you have any questions or inquires regarding our custom oligos or your order, please contact our product specialist at oligo@pentabase.com

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msi analysis


Custom Oligos



Synthesis scale

 

50 nmol

200 nmol

1000 nmol



Average Yield

 

5 – 30 nmol

10 – 80 nmol

30 – 200 nmol



Guaranteed yield (HPLC purification)

(Probes)

5 nmol

15 nmol

50 nmol



Guaranteed yield (HPLC purification)

(Primers)

5 nmol

10 nmol

30 nmol



Guaranteed yield (RPLC purification)

 

10 nmol

25 nmol

100 nmol

 



Fluorophores

5’ modifications

  • 5’ Biotin
  • 5’ phosphate
  • Amino-modifier-PRO
  • AquaPhluor 593
  • ATTO 390
  • ATTO 425
  • ATTO 488
  • ATTO 532
  • ATTO 550
  • ATTO 565
  • ATTO 590


  • ATTO 647
  • BHQ 1
  • BHQ 2
  • CAL Fluor Gold 540
  • CAL Fluor Orange 560
  • CAL Fluor Red 610
  • Cy3
  • Cy5
  • 5
  • DyLight 647
  • FAM


  • HEX
  • JOE
  • PentaAltRed
  • PentaGreen
  • PentaLightOrange
  • PentaLightYellow
  • PentaOrange
  • PentaPink
  • PentaPurple
  • PentaRed
  • PentaYellow


  • Quasar 570
  • Quasar 670
  • Quasar 705
  • Redmond Red
  • Rox
  • SIMA
  • Tamra
  • TET
  • Texas-RED – through amino linker
  • TFA-Amino-modifier-11-CE
  • Thiol-modifier C6 S-S

5’ modifications
3’ modifications

  • 3’ Biotin
  • 3’ FAM
  • BBQ-650
  • BHQ 1
  • BHQ 2
  • BHQ 3
  • Dabcyk
  • Green Quencher


  • Orange Quencher
  • Pink Quencher
  • Purple Quencher
  • Red Quencher
  • Tamra
  • Thiol C3 SS
  • Thiol C6 SS
  • Yellow Quencher



 

 

 

 

 

 

3’ modifications
Random Hexamer Primers

Random Hexamer Primers are a mixture of oligonucleotides representing all possible 6 bp sequence combinations (dN6).

Random Hexamer Primers consist of a mixture of oligonucleotides representing all possible hexamer sequences. Random Hexamer Primers are commonly used for priming single-stranded DNA or RNA for an extension by DNA polymerases or reverse transcriptases.

During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between mRNA and other RNA species present in the reaction.

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Laboratory analysis at PentaBase
Technology

All of our products can be made with our proprietary and innovative INA™ technology that allows us to create oligonucleotides and oligonucleotide-based assays that are more robust with increased affinity, specificity and sensitivity compared to products based on standard DNA chemistry.
Our products are compatible with standard PCR and real-time qPCR instruments. Our INA™ technology is applicable to a wide range of oligonucleotide types including DNA oligos, PCR primers and probes, blocking oligonucleotides and NGS adapters. We guarantee superior quality and performance of our INA™ modified oligonucleotides.

NGS ADAPTERS

BASEBLOCKERS™

(SU)PRIMERS

FLUORESCENT PROBES

EASYBEACONS™ & HYDROLEASY™ PROBES

BASEPROBES™

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lab analysis focus


EasyBeacon™ & HydrolEasy™ probes

PentaBase is offering high-quality, dual-labelled INA® modified probes with a broad range of different fluorophores and quenchers. We offer two types of proprietary, dual-labelled probes for real-time PCR; EasyBeacon™ probes for end-point analysis and hydrolyzable HydrolEasy™ probes for qPCR with the improved signal-to-noise ratio.

 

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Intercalating Nucleic Acid®

PentaBase’s proprietary technology, Intercalating Nucleic Acid® (INA®), is a hydrophobic base analog that is incorporated into oligonucleotides without replacing any nucleotide in the oligonucleotide sequence. The integrated INA® enhances the stacking effect of the DNA helix, which increases the affinity, sensitivity, and specificity of the oligonucleotide.

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BaseProbes™

BaseProbes™ is a universal master mix comprising one to four qPCR probes. The system combines the versatility of SYBRTM Green type detection with the multiplex capacity of molecular probes.

 

 

 

 

 

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FLUORESCENT PROBES

PentaBase is offering a high quality dual-labelled DNA probes and improved DNA probes with a broad range of different quenchers and fluorophores.

 

 

 

 

 

 

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NGS Adapters

Indexed PentAdapters™ for next-generation sequencing on Illumina and Ion Torrent sequencing platforms. Using PentaBase’s outperforming technologies, we have made indexed adapters ready-to-use and ready-to-ship, for the sensitive sequencing of multiplexed samples.

 

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BaseBlockers™

BaseBlockers™ are short oligonucleotides modified with several pentabases to ensure tight and specific binding to a sequence that should be inhibited from working as a template. They are furthermore modified to ensure that they do not work as potential primers.

 

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(Su)Primers

SuPrimers™ are DNA primers modified with our proprietary pentabases and compatible with all applications of your standard primers. SuPrimers™ can be used with all your standard reagents.

 

 

 

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Fluorescent probes

PentaBase is offering a high quality dual-labelled DNA probes and improved DNA probes with a broad range of different quenchers and fluorophores.

 

 

 

 

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PentAdapters™

Indexed PentAdapters™ for Next Generation Sequencing on Illumina and Ion Torrent sequencing platforms. Using our outperforming technologies, we have made indexed adapters ready-to-use and ready-to-ship, for the sensitive sequencing of multiplexed samples. Our portfolio includes both single and dual-indexed adapters, and we also produce customized and molecular adapters upon request.

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Oligonucleotides

Are you interested in our oligos? Find a local distributor, become our distributor or oligo at PentaBase directly.

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