SuPrimers™ are DNA primers modified with our proprietary NA® technology and compatible with all applications of your standard primers. The primers are based on standard DNA nucleotides, added one or a few pentabase modifications.
SuPrimers™ are DNA primers modified with our proprietary PentaBases and compatible with all applications of your standard primers. SuPrimers™ can be used with all your standard reagents. Adding PentaBases to your primer will improve one or more of the following features:
Affinity: Adding a PentaBase to an oligonucleotide increases affinity towards DNA. This means that your primer can work at higher temperatures.
Sensitivity: PentaBases increase the on-target portion of an oligo and thereby the sensitivity of the primer.
Primer-dimer: Adding a PentaBase to or near the 5′-end of an oligo, will increase the interaction of the 5′ ends of unbound primers. This reduces the tendency to 3′-end interactions needed to make primer-dimer. Hence, the result is reduced primer-dimer formation
Specificity: A SuPrimer can be made shorter than a standard primer and thereby be more specific in its binding to a target sequence. However, please note that the primer sequence should still need to be unique in order not to mis-prime.
Random Hexamer Primers are a mixture of oligonucleotides representing all possible 6 bp sequence combinations (dN6).
Random Hexamer Primers consist of a mixture of oligonucleotides representing all possible hexamer sequences. Random Hexamer Primers are commonly used for priming single-stranded DNA or RNA for an extension by DNA polymerases or reverse transcriptases.
During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between mRNA and other RNA species present in the reaction.
Applications for Random Hexamer Primers:
DNA synthesis using Klenow fragment with DNA templates
DNA probe synthesis for use in Northern and Southern blots, and in situ hybridization applications
Partially degraded RNA samples
RNA without poly(A) tail such as ribosomal RNAs
RNA with strong secondary structure
Target regions at 5´ end of a long messenger RNA transcript
