SuPrimer™ Oligonucleotides
SuPrimers™ are standard DNA primers added a single IPN molecule in the 5′ end. The modified SuPrimer™ works as a normal primer in the PCR reaction – just better. The addition of a hydrophobic IPN molecule reduces the ability to form primer dimers and at the same time increases target affinity and specificity. The result is more sensitive PCR amplification and higher fluorescent signals.
SuPrimer™ features and advantages include:
- Reduced primer-dimer formation
- Higher target affinity and specificity
- Higher assay fluoresence
- Reduced off-target amplification
Related Files
Reduced Primer-dimer Formation
Amplification using regular primers often results in unspecific products due to primer-dimer interactions and/or other off target events. Since SuPrimers are more hydrophobic due to the addition of an IPN molecule, they are less prone to form primer-dimers. The result is more specific amplification of the desired target sequence and reduced generation of PCR products related to primer-primer interactions.
Higher Fluorescence Levels
The reduced off-target binding of SuPrimers™ and higher affinity for the target leads to better priming of the PCR reaction and higher fluorescence levels.
