BaseBlockers™
BaseBlockers™ are short INA® oligonucleotides that are highly modified with Intercalating pseudo-nucleotides (IPNs). BaseBlockers™ have extremely high target affinity and specificity which makes them useful for target-specific blocking of PCR amplification.
Binary binding dynamics
BaseBlockers™are otherwise unmodified INA® oligos containing particularly many IPN molecules that provides BaseBlockers™ with an etremely high target affinity and specificity. BaseBlockers™ are furthermore modified to ensure that they do not work as potential primers.
The resulting target vs. off-target binding dynamics og BaseBlockers™ resemble a binary response even with single nucleotide variation between target and off-target sequences. This extreme sensitivity of BaseBlocksers™ to target sequence variation can be utilised to prevent binding of e.g. allel-specific primers to wild type sequences.
BaseBlockers™ are particularly useful in PCR assays for detection of low frequency mutations where selective amplification can be highly improved by including a BaseBlocker™ that specifically binds and blocks amplification of the wild type sequence. The addition of a BaseBlocker™ thereby lowers the risk of false positive results without influencing the rate of false negatives.
- Extremely high target affinity and specifity
- Sequence specific blocking of PCR amplification
- Reduced false positive PCR assay results
- Partucularily useful in low frequency SNV detection assays
Specifications
Nucleotides (without IPNs)
Available Yields (nmol delivered)
Publications
