BaseBlockers™ are short oligonucleotides modified with several pentabases to ensure tight and specific binding to a sequence that should be inhibited from working as a template. They are furthermore modified to ensure that they do not work as potential primers.
BaseBlockers™ are short oligonucleotides modified with several Pentabases to ensure tight and specific binding to a sequence that should be inhibited from working as a template. They are modified to ensure they do not work as potential primers. Hence, by adding BaseBlockers™, one can avoid false positive and negative signals.BaseBlockers™ work by binding strongly and specifically to one allele (for example, to a wild-type allele) while allowing amplification of the other allele. A BaseBlocker™ binds to an entirely complementary sequence, thereby inhibiting the primer from binding and amplifying the template.
Key features of BaseBlockers™
Very efficient in removing false positive signals
Little to no impact on mutation signals
Can remove cross signals
Significantly increases the robustness of an assay
Increases the detection range of your target
SNV detection assay using a green fluorescent HydrolEasy™ probe (light green), allele-specific forward primers (black), and a BaseBlocker™ (red). The allele-specific forward primers include the sequence variation in the 3´-end (green) and an additional sequence variation close to the 3´-end (purple). The BaseBlocker™ is complementary to the wild-type sequence (yellow) and specifically blocks amplification of the wild-type allele.
