Phone: +45 36 96 94 96 Mail: info@pentabase.com
  • Contact
  • FAQ
  • Distributors
  • Ressource
  • Product Catalogue
  • Book Test
  • Products
    • Assays
    • Instruments
  • Technology and services
    • Our proprietary technology
    • Custom Oligo
    • Baseblockers™
    • PentAdapters™
    • Fluorescent Probes
  • About Us
  • Services
    • SNP Genotyping Services
    • Real-Time PCR Assay Development
  • About
    • Meet the team
    • Career
    • News
    • Sponsorship
  • Services
  • Ordering
  • Support
  • Privacy Policy

Latest news

Friday 12. Aug 2022 by Aminat Makhauri

InfectionDetect-MPOX qPCR assay – A Danish developed qPCR assay for Monkeypox

Friday 29. Jul 2022 by Aminat Makhauri

MicroSight® MSI 1-step HRM Analysis for Microsatellite Instability

  • Contact
  • FAQ
  • Distributors
  • Ressource
  • Product Catalogue
Cart Cart

No products in the cart.

Menu

Design uden navn (34)



SHORT MODIFIED OLIGONUCLEOTIDES

Baseblockers™

BaseBlockers™ are short oligonucleotides modified with several pentabases to ensure tight and specific binding to a sequence that should be inhibited from working as a template. They are furthermore modified to ensure that they do not work as potential primers.

Order here
INTRODUCTION

BaseBlockers™ are short oligonucleotides modified with several Pentabases to ensure tight and specific binding to a sequence that should be inhibited from working as a template. They are modified to ensure they do not work as potential primers. Hence, by adding BaseBlockers™, one can avoid false positive and negative signals.BaseBlockers™ work by binding strongly and specifically to one allele (for example, to a wild-type allele) while allowing amplification of the other allele. A BaseBlocker™ binds to an entirely complementary sequence, thereby inhibiting the primer from binding and amplifying the template.

Key features of BaseBlockers™ 

Very efficient in removing false positive signals

Little to no impact on mutation signals

Can remove cross signals

Significantly increases the robustness of an assay

Increases the detection range of your target

BASEBLOCKER™ ASSAY PRINCIPLE

SNV detection assay using a green fluorescent HydrolEasy™ probe (light green), allele-specific forward primers (black), and a BaseBlocker™ (red). The allele-specific forward primers include the sequence variation in the 3´-end (green) and an additional sequence variation close to the 3´-end (purple). The BaseBlocker™ is complementary to the wild-type sequence (yellow) and specifically blocks amplification of the wild-type allele.

Benefits of BASEBLOCKER™

Adding BaseBlockers™ to your assay not only reduces the false positive signals from the presence of WT templates. BaseBlockers™ also reduces the demand for stringency, allowing the assay to be conducted on different instruments, in other laboratories, and by different operators. Furthermore, it increases the detection range of your target of interest. That is why we call BaseBlockers™ a universal PCR specifier.
BaseBlockers™ are customized to your assay and can be purchased in 50, 200, or 1,000 nmol synthesis scales. Just design an oligo spanning the site you need to block with the same affinity as your primer and ask for a BaseBlocker™.

The figure shows how a well-designed BaseBlocker™ can inhibit the false positive signal generated by a WT template with no influence on the mutation-specific assay.

Sponsor and collaborator
How to order

Custom Oligos

Are you interested in our BaseBlockers™?Find a local distributor, become our distributor, or order directly at PentaBase.

Order with PentaBase Order from your local Distributor Become a Distributor
hounö logo
facebook linkedin twitter

About

  • About Us
  • Meet the team
  • News
  • Career
  • Sponsorships

Covid-19

  • Book Test
  • COVID-19 for Companies

Assays & Instruments

  • Cancer Stratification and Monitoring
  • CoviDetect™ Variants Assay
  • CoviDetect™ FAST Assay
  • CoviDetect™ Assay
  • Oligonucleotides
© Copyright 2023 Pentabase A/S CVR: 30197852 Petersmindevej 1A DK-5000 Odense C, Denmark
  • Privacy Policy
  • Terms of payment
  • Cookie Policy