BaseBlockers™
BaseBlockers™ are short INA® oligonucleotides that are highly modified with Intercalating pseudo-nucleotides (IPNs). BaseBlockers™ have extremely high target affinity and specificity which makes them useful for target-specific blocking of PCR amplification.
Binary binding dynamics
BaseBlockers™ are otherwise unmodified INA® oligos containing particularly many IPN molecules that provides BaseBlockers™ with an etremely high target affinity and specificity. Our BaseBlockers™ include modifications that prevent them from acting as unintended primers.
The resulting target vs. off-target binding dynamics og BaseBlockers™ resemble a binary response even with single nucleotide variation between target and off-target sequences. This extreme sensitivity of BaseBlocksers™ to target sequence variation can be utilised to prevent binding of e.g. allel-specific primers to wild type sequences.
BaseBlockers™ enhance PCR assays for low-frequency mutation detection by selectively binding to the wild-type sequence and blocking its amplification. The addition of a BaseBlocker™ thereby lowers the risk of false positive results without influencing the rate of false negatives.
- Extremely high target affinity and specifity
- Sequence specific blocking of PCR amplification
- Reduced false positive PCR assay results
- Partucularily useful in low frequency SNV detection assays
Specifications
Nucleotides (without IPNs)
Available Yields (nmol delivered)
