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COVID-19

SARS-CoV-2 Oligonucleotides

1. SARS-CoV-2 Oligonucleotides 2. SuPrimers™ 3. HydrolEasy™ Probes

PentaBase is manufacturing oligonucleotides based on standard and proprietary DNA technologies

We are currently supplying our customers with oligonucleotides for SARS-CoV-2 PCR assays based on our proprietary INA® technologies including SuPrimers™ and HydrolEasy™ probes.

Standard DNA Primers 

SuPrimers™

Post PCR melt analysis of SARS-CoV-2 PCR amplicons generated using standard DNA primers (left) and SuPrimers™ (right)

SuPrimers™

SuPrimers™ are PCR primers modified with our proprietary technologies. The benefits of replacing primers based on standard DNA with SuPrimers™ include increased signal-to-noise ratio as well as increased sensitivity and specificity towards their target sequences

Real-time PCR amplification plots of SARS-CoV-2 PCR assay with standard DNA primers (red lines), one SuPrimer™ and one standard primer (blue lines), and two SuPrimers™ (green lines).

HydrolEasy™ Probes

HydrolEasy™ probes are dual-labelled hydrolysis probes based on our proprietary INA® technologies. HydrolEasy™ probes provide an increased signal-to-noise ratio and increased specificity and sensitivity toward the target sequence compared to standard TaqMan hydrolysis probes.

Standard DNA Primers and TaqMan Probe

Standard DNA Primers and HydrolEasy™ Probe

Real-time PCR amplification plots of SARS-CoV-2 assay with standard DNA primers and TaqMan probe (left) and standard DNA primers and HydrolEasy™ probe (right)

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