As one of the world's leading experts in sensitive real-time PCR, we have developed sensitive and specific RT qPCR assays for the detection of SARS-CoV-2 and mutations quickly, sensitively, and selectively.

PentaBase’s CoviDetect™ are In Vitro Diagnostic COVID-19 Real-Time RT (Reverse transcriptase) qPCR assays for the detection of COVID-19 and variants. The assays are an innovative method to detect COVID-19 on real-time PCR, which are based on WHO and CDC standards.
Available in the following formats:
Dispense Ready (DR)
Ready-To-Use (RTU)

CoviDetect™ Variants COVID-19 RT-PCR Assays are real-time RT (reverse transcription) COVID-19 Mutation assays intended for the detection of genetic variations from positive SARS-CoV-2 samples. Genetic variants (see list below) of SARS-CoV-2 RNA can be found in the liquid from the upper or lower respiratory tracts of infected individuals. Samples can be obtained by nasopharyngeal or oropharyngeal swabs or from saliva. Note that infection with any variant of SARS-CoV-2 can occur without showing any symptoms.
Our CoviDetect™ assays available for the detection of genetic variants of SARS-CoV-2 are provided in the following formats:
Multiplex
4-plex α β γ
4-plex β γ δ
4-plex (Delta and Delta Plus)

Genetic Variations included in CoviDetect™ Variants COVID-19 RT-PCR Assay
N501Y
P681H
P681R
Del69-70
E484K
E484Q
N439K
A701V
K417N
Y453F
F476L
V553L
L18F
Del242-244
S477N
A222V
H655Y
Q677H
L452R
T732A
V483F
T478K
DelY144
W152C
PentaBase’s CoviDetect™ FAST diagnostic assay is a Real-Time RT (Reverse transcriptase) qPCR assay based on highly sensitive technology used to detect SARS-CoV-2 virus in patients. The Multiplex assay is a combination of three specific genes for SARS-CoV-2 and an internal control for validating the method. Analysing the three specific SARS-CoV-2 genes result in minimising the risk of false-positive results. The use of CoviDetect™ FAST assay for detecting the RNA presence of the virus will provide a quick and sensitive Nucleic Acid Extraction and Real-Time RT-qPCR analysis in less than an hour.
A fast SARS-CoV-2 test with CoviDetect™ FAST with a total instrument time of 51 minutes with 16 minutes in the BasePurifier™ (Nucleic Acid Extraction Instrument and 35 minutes to run on the BaseTyper™ (Real-Time PCR Instrument).
How do we achieve this?
At PentaBase, we have optimised every step in the laboratory procedure to offer a new assay that provides a faster and more sensitive test result. The Limit of Detection (LoD) is among the best of its class that secures a market-leading sensitivity with an LoD of 5 copies and increased multiplexing of three viral genes of the SARS-CoV-2 virus.
Less instrument time
Higher Sensitivity with an LoD of 5 copies
Amplification of three viral genes of SARS-CoV-2 virus
Based on WHO and CDC guidelines
As either Dispense Ready (DR) or Ready-to-use (RTU) versions

PentaBase’s CoviDetect™ diagnostic assay is a COVID-19 Multiplex Real-Time RT (Reverse transcriptase) qPCR assay for the detection of COVID-19. It enables the analysis of three times as many samples with the use of the same standard laboratory equipment. This provides a reduced risk of false-positive results due to the improved target sensitivity and specificity compared with our standard PCR assays.
The CoviDetect™ assay allows the amplification of Viral N1 and N2 genes, and the human RNA internal control in a multiplex reaction with a single tube. The test capacity increases while the dependency on consumables is reduced.

COVIDETECT™ COVID-19 MULTIPLEX RT-QPCR N1 ASSAY COMPARISON TO COMPARATOR METHOD.
COVIDETECT™ COVID-19 MULTIPLEX RT-QPCR N2 ASSAY COMPARISON TO COMPARATOR METHOD.
Correlation between CoviDetect™ COVID-19 Multiplex RT-qPCR N1 and N2 assay Ct values and Comparator Method using SARS-CoV-2 positive leftover oropharyngeal swab specimens
The CoviDetect™ FAST COVID-19 Multiplex RT-qPCR Assay is designed to run on open platforms and, currently, it has been validated on BaseTyper™ (PentaBase), and CFX96 (BioRad) Real-Time PCR instruments.
The CoviDetect™ Variants COVID-19 RT-PCR Assay is designed and validated to be used on open Real-Time PCR platforms and equipment such as BaseTyper™ (PentaBase PCR instrument), Biorad CFX and Thermo Fisher Scientific QuantStudio5.
The CoviDetect™ COVID-19 Multiplex RT-qPCR Assay is designed and validated to be used on open Real-Time PCR platforms and equipment such as BaseTyper™ (our PCR instrument), LightCycler 480 II, ABI 7500™ and ViiA7™ for detecting FAM, HEX/VIC, and Cy5 channels.


Order our CoviDetect™ assays
Are you interested in our assays for the detection of SARS-CoV-2 and variants? Find a local distributor, become our distributor or order your assay at PentaBase directly.