As one of the world's leading experts in sensitive real-time PCR, we have developed sensitive and specific RT qPCR assays for the detection of SARS-CoV-2 and mutations quickly, sensitively, and selectively.
PentaBase’s CoviDetect™ FAST diagnostic assay is a Real-Time RT (Reverse transcriptase) qPCR assay based on highly sensitive technology used to detect SARS-CoV-2 virus in patients. The Multiplex assay is a combination of three specific genes for SARS-CoV-2 and an internal control for validating the method. Analysing the three specific SARS-CoV-2 genes result in minimising the risk of false-positive results. The use of CoviDetect™ FAST assay for detecting the RNA presence of the virus will provide a quick and sensitive Nucleic Acid Extraction and Real-Time RT-qPCR analysis in less than an hour.
A fast SARS-CoV-2 test with CoviDetect™ FAST with a total instrument time of 51 minutes with 16 minutes in the BasePurifier™ (Nucleic Acid Extraction Instrument and 35 minutes to run on the BaseTyper™ (Real-Time PCR Instrument).
How do we achieve this?
At PentaBase, we have optimised every step in the laboratory procedure to offer a new assay that provides a faster and more sensitive test result. The Limit of Detection (LoD) is among the best of its class that secures a market-leading sensitivity with an LoD of 5 copies and increased multiplexing of three viral genes of the SARS-CoV-2 virus.
Less instrument time
Higher Sensitivity with an LoD of 5 copies
Amplification of three viral genes of SARS-CoV-2 virus
Based on WHO and CDC guidelines
As either Dispense Ready (DR) or Ready-to-use (RTU) versions
The CoviDetect™ FAST COVID-19 Multiplex RT-qPCR Assay is designed to run on open platforms and, currently, it has been validated on BaseTyper™ (PentaBase), and CFX96 (BioRad) Real-Time PCR instruments.
The CoviDetect™ Variants COVID-19 RT-PCR Assay is designed and validated to be used on open Real-Time PCR platforms and equipment such as BaseTyper™ (PentaBase PCR instrument), Biorad CFX and Thermo Fisher Scientific QuantStudio5.
The CoviDetect™ COVID-19 Multiplex RT-qPCR Assay is designed and validated to be used on open Real-Time PCR platforms and equipment such as BaseTyper™ (our PCR instrument), LightCycler 480 II, ABI 7500™ and ViiA7™ for detecting FAM, HEX/VIC, and Cy5 channels.